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STEMCELL Technologies Inc gentle cell dissociation medium 7174
Gentle Cell Dissociation Medium 7174, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gentle+cell+dissociation+medium+7174/gentle+cell+dissociation+medium+7174/pm29902441-213-12-15
Average 90 stars, based on 1 article reviews
gentle cell dissociation medium 7174 - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Gut Microbiota Produced Tryptamine Activates an Epithelial G-protein Coupled Receptor to Increase Colonic Secretion
Article Snippet: The resulting clean colon tissue was minced into ~1mm 2 pieces and washed again with cold Ca 2+ and Mg 2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for fifteen minutes in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70μm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4°C for 5 minutes.

Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: The resulting clean colon tissue was minced into 1mm2 pieces and washed again with cold Ca2+ and Mg2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for 15 min in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70mm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4 C for 5 min.

Gentle:

Article Title: Gut Microbiota Produced Tryptamine Activates an Epithelial G-protein Coupled Receptor to Increase Colonic Secretion
Article Snippet: The resulting clean colon tissue was minced into ~1mm 2 pieces and washed again with cold Ca 2+ and Mg 2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for fifteen minutes in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70μm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4°C for 5 minutes.

Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: The resulting clean colon tissue was minced into 1mm2 pieces and washed again with cold Ca2+ and Mg2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for 15 min in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70mm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4 C for 5 min.

Transferring:

Article Title: Gut Microbiota Produced Tryptamine Activates an Epithelial G-protein Coupled Receptor to Increase Colonic Secretion
Article Snippet: The resulting clean colon tissue was minced into ~1mm 2 pieces and washed again with cold Ca 2+ and Mg 2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for fifteen minutes in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70μm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4°C for 5 minutes.

Article Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Article Snippet: The resulting clean colon tissue was minced into 1mm2 pieces and washed again with cold Ca2+ and Mg2+ free PBS (Corning; 21-040-CV) until the supernatant was clear of any luminal debris. .. The minced tissue pieces were then incubated for 15 min in gentle cell dissociation medium (Stem cell technologies, 7174) and then rinsed vigorously using a 10mL Pasteur pipette pre-wet with freshly prepared filter-sterilized 0.1% PBS-BSA solution to prevent tissues from sticking to the pipette. .. The tissue suspension was filtered through a 70mm nylon mesh (Fisher Scientific, 22363548), and filtrate containing the crypt stem cells were centrifuged in a 15mL conical at 290 g at 4 C for 5 min.



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STEMCELL Technologies Inc gentle cell dissociation medium 7174
Gentle Cell Dissociation Medium 7174, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gentle+cell+dissociation+medium+7174/gentle+cell+dissociation+medium+7174/pm29902441-213-12-15
Average 90 stars, based on 1 article reviews
gentle cell dissociation medium 7174 - by Bioz Stars, 2026-09
90/100 stars
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